The American favorite Built Ford Tough Ford Ranger is poised for even wilder adventure as it builds on a legacy of customization with more than 150 performance parts and accessories that will be available when the truck launches early next year. The Ford Performance and Ford Accessories teams are helping to prepare Ranger to shine at SEMA with two of seven customized trucks featured at the Ford display. The Ford Performance off-road Ranger is a capable trekker featuring performance parts and accessories geared to moving quickly even when the pavement ends. The Ford Accessories Base Camp Ranger highlights how the Ranger’s extreme flexibility can be enhanced further for camping customers and those looking to get off the grid for a getaway. With more than 50 vehicles on exhibit, Ford will feature the largest original equipment manufacturer display at SEMA.
Axion Digital Photo Frame Owner's Manual Axion produced 8 Digital Photo Frame product models. The product manual is listed in the product model page. 8 manuals are published in devicemanuals.com.A free digital copy of the product manual is available for A.
And don’t miss the interactive experience from Ford Out Front, offering attendees a ride in a Ford Performance vehicle on a closed course. This Ranger Pre-Runner from Ford Performance scouts new trails A bold Pre-Runner from the Ford Performance group highlights Ranger’s go-anywhere, do-anything flexibility and capability that’s crucial for off-road teams to scout trails. It all begins with the FX4 Off-Road Package and adds a 3.73 electronic locking rear differential, Fox/BDS Suspension three-inch leveling kit with off-road shocks, BDS upper control arm kit and Ford Performance cat-back exhaust, all riding on BFGoodrich ® All-Terrain KO2 tires mounted to 17-inch Ford Performance wheels. Off-road accessories include a Desert Racer front bumper with integrated skid plate and rear bumper, illuminated Rock Slider side steps and chase rack from Addictive Desert Designs ® with RIGID ® LED light bar, LED underbody lighting and off-road light kit. A Rotopax FuelpaX fuel can system and swing case from Undercover ™ provide added back-country range. Ranger Base Camp adventures anywhere, then comfortably rests the crew Ford Performance’s 2019 Ford Ranger Base Camp is ready to go off-road at a moment’s notice with a Fox/BDS Suspension three-inch leveling kit and off-road shocks, BDS upper control arm kit, skid plates and differential cover from Ford Performance. Ascent front and rear bumpers by WARN Industries plus a WARN 10k Zeon front winch and grille guard add protection.
An A-pillar snorkel from Ford Performance keeps the engine breathing, while 18-inch BFGoodrich All-Terrain KO2 tires on machined-face black wheels with silver and orange accents provide grip. RIGID LED lighting and TOUCHLINK bed light by Lumen keep everything illuminated. Ford Licensed Accessories by Yakima include a Skinny Warrior roof basket, heavy-duty rack kit by Outpost, Slim Shady awning, heavy-duty hook straps and recovery track mount. Interior upgrades include black chrome door sill plates and all-weather floor liners from Ford Accessories, along with a safe by Console Vault and a Ford Performance by WARN Industries recovery kit for added security. Adventure hard, game hard: Xbox Ranger from Addictive Desert Designs Addictive Desert Design’s 2019 Xbox Ranger may be the closest thing to bringing video games to life.
This truck’s colors and modern camo pattern were inspired by, a program where gamers can customize their very own Xbox Wireless Controllers. Thanks to a collaboration with one of the Forza racing franchise’s most prolific gamers, Nick Andrew aka AR12 Gaming, passengers can game with two rear-seat, custom-mounted Xbox One Xs. Uniquely, they can then pipe the gameplay to screens in the bed of the truck to game under the stars at the campsite after a long day exploring.
For adventuring, the truck features BDS Suspension upper control arms and lift kit and 17-inch Rev 883 wheels, while BFGoodrich Mud Terrain KM3 tires provide the stance. Addictive Desert Designs’ front and rear bumper, HoneyBadger chase rack with tire carrier and RIGID LED lighting provide the style. Everything is topped off with an Xbox Design Labs winter forces-themed vinyl wrap.
Project Nightfall is an adventurer that’s equally at home in the city Project Nightfall combines off-road capability with sleek, upscale styling that makes it equally fit for urban living. Accessories begin with an A.R.E. CX Revo cap, BDS Suspension upper control arms, Airlift load lifter, Fox shocks, Bushwacker fender flares, custom Ford Performance stealth bumpers, NFab Epyx side steps and Black Rhino Mint 20-inch wheels with Cooper Discoverer STT Pro tires for a smooth silhouette. Custom Axalta paint by Lynch Concepts immediately grabs the spotlight. Wrapped in a cool matte gray with vivid green highlights, Project Nightfall is the perfect complement to Ford Ranger’s return to the North American market. Base vehicle.
2019 Ranger Lariat 4x4 SuperCrew. 2.3-liter EcoBoost I4 engine. 10-speed automatic transmission Powertrain.
Ford Performance by Borla cat-back exhaust and differential cover Chassis. BDS Suspension upper control arms. Airlift load lifter.
FOX 3-inch leveling kit with off-road shocks Wheels and tires. Black Rhino Mint wheels, 20 x 10 inches. Cooper Discoverer STT Pro tires, 305/55R-20 Exterior. Custom stealth bumpers from Ford Performance. A.R.E. Project Ranger X from BDS Suspension has the X-factor for more performance, capability and utility Project Ranger X takes Ranger’s built-in performance and capability to the next level in the form of this ultimate back-country adventurer.
The truck features a custom long-bed conversion and Knapheide utility box with custom-built rack system for tools, supplies and recovery gear. Ranger X is ready to do battle with Mother Nature with a BDS Suspension six-inch suspension lift kit with upper control arms, Fox 2.5 Factory Series DSC coil-overs, AirLift rear airbag system and KMC XD Series Grenade off-road wheels wearing BFGoodrich Mud Terrain KM3 tires. A WARN Ascent front bumper with Zeon 10-S winch, rock sliders and full underbody skid plates keep the truck protected out on the trail. Ranger X is ready to tackle the deepest wilderness and the most extreme adventures. Camo conquering: Baja-forged Ranger’s off-road prowess lives up to its military style This Baja-forged Ranger has a military-tough persona with a clean, purposeful off-road design.
And it’s ready for duty with signature Baja-forged tube-style front and rear bumpers, bed cage with Pro Eagle jack, rock rail and fender flares. Its aggressive stance is complemented by an upgraded mid-travel suspension and lift from Icon Vehicle Dynamics. Everything rides on a set of BFGoodrich Mud Terrain KM3s on 17-inch bronze wheels from Icon that are ready to tackle the terrain. An army green paint scheme with bronze and yellow accents makes for a tough-edged design that commands attention.
Ford F-Series, America’s best-selling truck, are making a bold Built Ford Tough statement at SEMA by maximizing off-road performance, blazing new trails of adventure and delivering pure raw power. The possibilities seem endless as Ford NASCAR drivers, country music stars and famed tuners bring distinctive style and capability F-150 and Super Duty. With more than 50 vehicles on display, Ford will feature the largest original equipment manufacturer exhibit at SEMA. Plus, Ford Out Front will offer attendees an interactive experience of riding in a performance car or truck on a closed course. Kurt Busch and ZB Customs 1000-hp 2018 F-150 Lariat Sport Cranks Power, Tunes and Hot Laps This 2018 Ford F-150 Lariat Sport 4x2 SuperCab by ZB Customs and Kurt Busch is a 1,000-horsepower cruiser designed to shred tires and turn heads wherever it goes.
The force-fed 5.0-liter Ford V8, it has On3 twin turbochargers, JE forged pistons, Ford Performance forged rotating assembly, oil pan and pump and a side-exit exhaust. ZB Customs added its 4.5-inch wide body fenders, bumpers and grille insert, plus a DezignWorks carbon spoiler and splitter and Axalta Electron Blue custom pearl ghost graphics. Inside, ZB Customs installed alligator leather seats, steering wheel and armrests. A Ford Accessories subwoofer by KICKER Audio puts the finishing touches on this show-stopper F-150. Gen-2 Full-Race Motorsports ‘Freak-O-Boost’ blends a vision for hardcore F-150 EcoBoost performance with go-anywhere, weekend warrior outdoor utility Full-Race Motorsports’ Gen-2 “Freak-O-Boost” F-150 pickup is loud, proud and sure to draw a crowd. It starts with Ford’s 3.5-liter EcoBoost V6 engine with Full-Race twin turbo upgrade, CARB approved intercooler, cold-air intake, charge pipes and cat-back exhaust.
![Dpf Dpf](http://www.mediafire.com/imgbnc.php/e4eb13f3afbd565542b10aee26d6e926c25abb26e8a3e2533e37bc6314ae0e7d4g.jpg)
ICON Vehicle Dynamics springs, suspension and 17-inch bead-lock wheels help put the power to the dirt. Outside, the F-150 wears Fiberwerx 4.5-inch wide body fenders and bedsides, Rogue Racing front/rear Enforcer bumpers, Ford Performance side steps and Maxxis RAZR MT tires. A bold orange and blue paint scheme with custom Morimoto projector headlamps and LED lighting from Rigid are the finishing touches. Transfer Flow builds the ultimate family adventure pickup, pairing the power of F-150 with the latest off-road upgrades and accessories California-based Transfer Flow has created the ultimate family Ford F-150 adventure truck that leverages the efficient power of the all-new 3.0-liter Ford Power Stroke ® diesel, taking it to new heights with off-road suspension, powertrain and accessory upgrades.
Add in Fab Fours Vengeance front and rear bumpers and roof rack, Yakima kayak rack, a Transfer Flow 50-gallon fuel tank and 40-gallon in-bed auxiliary toolbox combo with refueling kit and movie projector screen inset for maximum getaway fun. Extang teams with college students to build ultimate tailgate truck Michigan-based Extang Truck Bed Covers enlisted the help of local college students to help build this custom Ford F-350 Super Duty Lariat. The ultimate tailgate truck features a slide-out game table, cooler, BBQ grill and large-screen OLED television and sound system designed by Extang engineers and built by students from Washtenaw Community College’s Custom Cars and Concepts program. 2019 F-250 Super Duty off-road adventure truck by Wilderness Collective Wilderness Collective, a leading moto tours company, has leveraged its off-road experience to design its vision of the ultimate adventure Ford F-250 Super Duty pickup. The build begins with an Icon Stage 5 suspension lift kit with Hellwig Products air lift, steering upgrades and WARN Industries manual front hub-lockers.
These modifications make room for a massive set of Toyo Open Country M/T tires on 20-inch Fifteen52 TurboMac wheels. The team added a full-guard heavy-duty front bumper, M12 winch and rear ascent bumper from WARN Industries plus a cab and bed rack, rooftop tent, cargo system from Frontrunner, and RIGID light bar spotlight and floods. Power comes from the 6.7-liter Ford Power Stroke diesel with Ford Performance exhaust. Inside, the truck features a full recovery kit from WARN and a Rugged Radios communications system. “Which Way” map graphics were created by Justin Bauer of Wilderness Collective. Bauer will accompany the truck for its maiden trip across the desert en route to the show floor at SEMA.
2019 F-250 Super Duty Transformer Work Truck by DeBerti Design gets the job done right Massively strong, extremely capable and incredibly stylish, this F-250 Super Duty 4x4 Transformer Work Truck by DeBerti Design redefines what it means to be the boss. The 6.7-liter Power Stroke diesel has been modified with the addition of a twin-turbo kit from Midwest Diesel & Auto and Fleece Performance Engineering.
It features a No Limit Fabrication air-to-liquid intercooler, EGR kit and cold-air intake, and is finished off with a performance diesel tailpipe from Ford Performance. Proformance Diesel worked to dial it all in to crank out 900 horsepower. DeBerti Design added some prime cuts to the Super Duty suspension with Eibach coils, adjustable Fox shocks, Hellwig Products airbags, sway bars and a beefy set of BFGoodrich KR2 37-inch tires mounted to Ultra-Wheel beadlock wheels. The crew will arrive in comfort and style starting with a custom interior and fully custom-fabricated utility bed, pull-out bumper utility box, custom alloy grille and RIGID lights mounted to a light bar rack from DeBerti Design. Blood Type Racing’s Edge ST amps-up Ford Performance’s first SUV Ford is entering new territory with the Edge ST – the first SUV from the Ford Performance team – and now teams with Chicago-based Blood Type Racing to push the dynamic new entry even further into the performance space.
The team upgraded Edge ST’s stealthy design and twin-turbo V6 EcoBoost ® performance with a slammed suspension, its own performance induction, Mishimoto charged intercooler, and an MRT dual stainless performance exhaust to crank up the horses. Blood Type Racing finished it off with low-profile Toyo Tires ® STIII Proxes tires on 22-inch wheels, custom aerodynamics and a gloss blue wrap. Interior features include a SoCal carbon fiber steering wheel, Kicker Bullfrog ® portable audio, Ford Accessories all-weather floor mats and cargo area protector, tablet cradle and cooler bag. LGE.CTS Expedition Classic honors vintage off-roaders while offering modern technology With vintage design cues, rich materials and high-tech rugged hardware, designer/builder Theresa Contreras created a modern classic for SEMA with her LGE.CTS Expedition Classic.
From the purpose-driven matte paint and steel wheels, to the ReadyLIFT ® leveling suspension and Borla ® performance cat-back exhaust, the LGE.CTS Expedition Classic is built for adventure – and getting you there in ultimate style. Finishing touches include custom-painted Yakima OffGrid roof rack and coordinating Ford Accessories. Destination Expedition: Hulst Customs’ ultimate family adventurer This Hulst Customs 2018 Destination Expedition is the ultimate SUV – a custom luxe cruiser designed to get you to where you are going in comfort, class and style. A custom hood and grille, plus custom Borla cat-back exhaust with custom-fabricated tips give a nod to its performance DNA. Color-matched 26-inch Lexani wheels and tires fill out the fender wells, while stunning custom gray paint with lower graphics give this Destination Expedition a truly rich and distinctive appearance. A custom drop visor with LED running lights and Yakima snowboard rack put the finishing touches on this one-of-a-kind family hauler. MAD Industries Explorer blends style and athleticism California-based MAD Industries created a high-fashion design that blends style and performance with this custom Explorer.
The two-tone Explorer’s performance is upgraded with more aggressive handling tuning and more braking power. The interior features premium leather seats, custom MAD Industries steering wheel and unique touches including billet aluminum pedals, color-matched accents and LED lighting.
Tucci Hot Rods gives EcoSport go-anywhere, do-anything treatment This Tucci Hot Rods Big Adventures EcoSport gives an all-new meaning to the concept of compact sport utility vehicle. The team started with custom fender flares and beefy Maxxis Bravo AT tires, then followed with off-road accessories including an integrated winch, custom tubular bumpers, Rigid Industries lighting and Yakima bike rack. The result is a highly capable, efficient, fun package from an award-winning team that will turn heads and open the great outdoors to a new generation of compact SUV builders. Top tuners are at it again cranking up America’s favorite sports car even more for the 2018 Specialty Equipment Manufacturers Association Show, showcasing why Mustang is one of the world’s most customized vehicles. Having grown into an all-around global sports car, these five tuned Mustangs will see all performance ingredients enhance to help Mustang fans make a statement on nearly any track or street they choose.
With more than 50 vehicles on exhibit, Ford will feature the largest original equipment manufacturer display at SEMA. And don’t miss the interactive experience from Ford Out Front, offering attendees a ride in a Ford performance vehicle on a closed course and chances to meet racing personalities across various series.
Galpin Auto Sports takes Mustang GT to the track with its wide-body inspired road racer This Galpin Auto Sports (GAS) build boasts an aggressive yet sleek road racing inspired wide body design, with additional downforce measures incorporated into the front splitter, side rockers, and rear diffuser. The team included the very best Ford Performance parts to up the street cred with big horsepower and a full track-focused suspension.
Taunting “Smoke Show”: CJ Pony Parts 5.0-liter supercharged Drifting Mustang GT CJ Pony Parts brings an American tribute with a metallic twist for this 700-plus-horsepower 2018 Mustang GT. This thoroughbred features Stars and Stripes livery in custom dark shadow gray paint with copper accents including a Watson Racing roll cage in copper. The build showcases a Ford Performance supercharger and follows through with hard-core body and suspension upgrades to deliver a true drift beast. CGS Motorsports Mustang GT: Making sinister more tasteful CGS Motorsports brings tasteful modifications to a 2018 Mustang GT with this clean interpretation of modern muscle.
Large 410-millimeter rotors combine with six-piston Brembo calipers to deliver the stopping power necessary to properly tame this vicious steed. Nighttime driving in this supercharged Mustang is a well-illuminated affair thanks to unique LED lighting from a complete conversion by Diode Dynamics to Rigid undercar lighting.
Everyone from night owls to track rats can prowl or stalk tracks in this stylishly sinister Mustang. TJIN Edition EcoBoost Mustang takes turbo-charged fun to the next level This EcoBoost ®-powered 2018 Mustang by TJIN Edition manages to shout even when parked with hypnotic Electric Purple paint combined with rivet style custom bodywork and RTR parts. An adjustable air suspension provides a slammed look when desired while still showcasing the Forgeline center-lock 20’s wheels. Putting some serious beast in the EcoBoost comes courtesy of a turbo kit from Full-Race with upgrades by BorgWarner. Bojix Design Mustang GT brings both flash and dash Subtlety be damned, this flashy 2018 Mustang GT will draw crowds everywhere it goes even amongst the wild designs of the SEMA Show. Bojix brings us another showstopper in stimulating two-tone blue and gray with wide 20-inch Savini wheels putting the appeal to the pavement.
A rear seat-delete and upgraded aero elements emphasize this pony car is both show and go.
Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion.
Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation. Introduction Nitric oxide (NO) is a signalling molecule that regulates synaptogenesis in both central and peripheral nervous tissue –. This small, highly diffusible molecule, synthesized in biological tissues by a family of enzymes termed the nitric oxide synthases (NOS’s), mediates its effects principally via activation of soluble guanylyl cyclase (sGC) and cyclic guanosine monophosphate (cGMP) synthesis –. The NOS1 isozyme is expressed in neuronal tissue, often during periods of growth cone extension and synapse formation, – and can influence both synapse assembly – and maintenance,. Recent evidence also suggests NO has developmental effects on the developing neuromuscular junction (NMJ): in Xenopus and chick embryos chronic NO treatment promotes acetylcholine (ACh) receptor clustering –. In addition, acute exposure to NO donors depresses spontaneous and evoked synaptic transmission at the NMJ of developing amphibians, an effect which may contribute to activity-dependent maturation of neuromuscular synapses.
Moreover, work in our laboratory recently demonstrated that NO/cGMP signalling regulates arborisation of zebrafish spinal motoneurons. Here, NOS1 is observed in interneuron clusters that form near to motoneurons of the developing zebrafish spinal cord,. Developmental inhibition of NO/cGMP activity markedly increases the number of collaterals formed on motor axons over the first three days of development whereas exogenous exposure to either NO donors or cGMP analogs has the opposite effect. Whilst these observations strongly suggest that NO/cGMP signalling influences zebrafish motor axon development, the consequences to NMJ maturation remain poorly understood. The aim of the current study was to determine how NO signalling influences anatomical and physiological maturation of zebrafish NMJs.
Using histochemical approaches we show that developmental manipulation of NO and cGMP signalling affects the formation of NMJs along the axial swimming muscles of developing zebrafish. In addition, using in vivo patch clamp electrophysiology we show that developmental perturbation of NO affects the kinetics of spontaneous miniature end plate currents (mEPCs) at nascent NMJs. Finally, we provide evidence for NO-dependent effects on the maturation of locomotor network activity. Our data provides in vivo evidence that NO/cGMP signalling affects NMJ and locomotor maturation in zebrafish. Ethics and Zebrafish Care Zebrafish were maintained according to established procedures and in compliance with the Animals (Scientific Procedures) Act 1986.
Embryos were collected and incubated at 28.5°C in embryo medium until the required developmental stage. Staging was performed in accordance with Kimmel et al. All experiments were conducted on 2 day old zebrafish embryos. At this immature stage, zebrafish are not considered to be sentient and are unable to register pain. Thus ethics approval was not required for this study.
On completion of electrophysiology experiments, embryos were anaesthetised with 0.02% MS-222 and killed with a bleach solution (sodium hypochlorite 6.15%). For histochemistry experiments, embryos were anaesthetised with 0.02% MS-222 and subsequently euthanised with 4% paraformaldehyde (PFA, Fisher Scientific). Pharmacological Reagents During this study the following drugs were used: diethylenetriamine/nitric oxide adduct (DETA-NO, 250–500 µM, Sigma), Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME, 500 µM-1 mM, Sigma), 8-(4-Chlorophenylthio)-guanosine 3′, 5′-cyclic monophosphate sodium salt (8-pCPT-cGMP, 500–750 µM, Sigma), 1 H-, Oxadiazolo4,3-aquinoxalin-1-one (ODQ, 500 µM, Ascent Scientific), tetrodotoxin (TTX, 1 µM, Ascent Scientific), (+)-tubocurarine hydrochloride (tubocurarine, 3 µM, Sigma), formamide (2 M, Sigma) and 18-β-glycyrrhetinic acid (18βGA, 100 µM, Sigma). Chronic Drug Treatments To exogenously elevate NO and cGMP levels the NO-donor DETA-NO and the cGMP analog 8-pCPT-cGMP were diluted in embryo medium (pH 7.4) to the desired concentration.
Embryos at 24 hours post fertilisation (hpf) were placed in the resulting solution. Inhibition of NO or cGMP synthesis was achieved with L-NAME or ODQ, respectively. As these drugs appeared to exhibit low skin penetrance, they were dissolved in Evans physiological saline composition (in mM): 134 NaCl, 2.9 KCl, 2.1 CaCl 2, 1.2 MgCl2, and 10 HEPES, pH 7.8 containing fast green (0.2%; Sigma) and around 50 nl of the resulting solution was injected directly into the yolk. To do this, glass microinjection needles (tip diameter: 10–20 µm) were filled with drug solution and inserted into the yolk of 24 hpf embryos. Drugs were pressure-ejected using a Picospritzer microinjector (Parker, USA).
Subsequent to drug treatment, embryos were incubated at 28.5°C until 48–52 hpf. Labelling of Putative NMJs Histochemistry was performed as previously described. Briefly, fish were anaesthetised in 0.02% MS-222 (Acros Organics) and subsequently fixed in 4% PFA for 90 min at room temperature. After thorough rinsing in PBSTX (Phosphate buffered saline containing 0.1% Triton-X 100), fish were placed in PBS containing 1 mg/ml collagenase (Sigma) for 16 min. After rinsing in PBSTX, fish were placed in blocking solution (composition: 3% milk powder; 1% DMSO; 0.1% Triton-X 100 in PBS) containing 10 µg/ml rhodamine-conjugated α-bungarotoxin (Rh-α-BTX; Sigma) which labels postsynaptic ACh receptors. After 30 min the block/Rh-α-BTX solution was rinsed off and fish were placed in blocking solution containing anti-SV2 antibodies (1∶200; Developmental Studies Hybridoma Bank (DSHB), University of Iowa), which label presynaptic neurotransmitter vesicles.
Fish were incubated in primary antibody overnight at 4°C before thorough rinsing in PBSTX and incubation in fresh blocking solution. After 40 min AlexaFluor 488 secondary antibody (1∶500; Invitrogen) was added to the block solution and the fish were incubated overnight (4°C). Fish were subsequently rinsed with PBS and cleared in glycerol prior to mounting for image acquisition. Image Acquisition and Analysis of NMJ Distribution Labelled NMJs were imaged with an Olympus FV1000 confocal microscope connected to a PC running Fluoview FV1000 capture software. Images were captured using a 40x objective in z-stacks spaced at 3 µm increments.
Staining dorsal to the horizontal myoseptum was excluded from analysis. All experiments were conducted on fish ranging between 48 and 52 hpf and within each experimental replicate fish were carefully age-matched to minimise age-dependent variation. Images were obtained from trunk segments located at the level of the yolk-sac extension. Acquired z-stacks were compressed into a single image and de-convoluted using Huygens Essential software prior to analysis with the ImageJ plugin SynaptcountJ.
The accuracy of this method was confirmed via visual quantification of NMJ distribution. To analyse total NMJ number per somite, regions of interest (ROIs) were selected such that they spanned a single motor axon fascicle and its associated branches.
For fascicle or branch analysis, ROIs were selected to encompass either the fascicular or motor axon branch domains within each somitic region. To quantify the density of branch-associated NMJs, the total number of branch-associated puncta was divided by the sum of axon branch lengths.
Developmental elevation of NO/cGMP signalling decreases NMJ numbers. Left: schematic illustration of a zebrafish embryo at 2 days post fertilisation (dpf). Dashed box indicates region used for puncta analysis. Right: schematic illustration of a motoneuron at 2 dpf. Motoneuron somata (black circle) are located within the spinal cord (sc) and their axons extend along fascicles (solid black line) into the somitic region. Axon branches (solid grey lines) extend from the main fascicle.
Dashed lines indicate somitic boundaries B. Left hand panels: lateral trunk views of anti-SV2 (green)/Rh-α-BTX (red) co-staining in control (top) and DETA-NO treated (bottom) zebrafish at 2 dpf. Right hand panels: expanded regions showing staining localised to a single somitic region.
Insets show SV2 (upper) and Rh-α-BTX (lower) z-projections from which merged images were derived. Bar chart depicting the mean (± SEM) number of synapses located within each somite (som), along motor fascicles (fas) and along branch-associated regions (branch) of control (black) and DETA-NO (grey) treated fish. Mean density of branch-associated puncta in control (black) and DETA-NO treated (grey) fish. Left hand panels: lateral trunk views of anti-SV2 (green)/Rh-α-BTX (red) co-staining in control (top) and 8-pCPT-cGMP treated (cGMP, bottom) zebrafish at 2 dpf. Right hand panels: expanded regions showing staining localised to a single somitic region. Bar chart depicting the mean (± SEM) number of synapses located within each somite (som), along motor fascicles (fas) and along branches of control (black) and 8-pCPT-cGMP (cGMP, grey) treated fish.
Mean density of branch-associated puncta in control (black) and 8-pCPT-cGMP treated (cGMP, grey) fish. Scale bars = 30 µm. In vivo Patch Clamp Electrophysiology To prepare fish for physiological recording, 48–52 hpf embryos were anaesthetised in MS-222 (0.02%) and secured to a Sylgard-containing Petri dish via insertion of fine (0.025 mm) tungsten pins through the notochord. The trunk skin was carefully removed with a pair of fine forceps to expose the underlying muscle tissue. Fish were then transferred to a patch clamp microscope (Nikon FN-1) and the MS-222 washed off with Evans physiological saline containing 10 mM glucose.
To gain access to embryonic fast twitch fibres, a small number of overlying embryonic slow twitch fibres were removed from a two to four somite region via aspiration with a broken-tipped patch electrode. All recordings were made from ventral fibres in somites adjacent to the tip of the yolk extension; approximately the same position used for imaging of NMJs (see above). For all physiological experiments, whole cell patch clamp electrodes (resistance = 3–10 MΩ) were pulled from borosilicate glass (Harvard Apparatus, UK) using a P-80 micropipette puller (Sutter, USA). Electrodes were filled with a K-gluconate recording solution containing (in mM): 10 HEPES, 10 EGTA, 2 MgCl6 H 2O, 10 NaCl, 6 KCl and 126 D-gluconic acid-potassium salt (pH adjusted to 7.2 with KOH). During analysis of mEPCs, 1 µM TTX was added to the Evans physiological saline to ensure that recorded cells were synaptically isolated. For experiments in which mEPC kinetics were analysed, the gap junction blocker 18βGA (100 µM) was included in the bath solution in order to reduce contamination from electronically generated currents originating in neighbouring muscle fibres.
To study effects of acute DETA-NO/L-NAME application, fish were exposed to a 10–20 min pre-treatment with 2 M formamide prior to experimental recording. This treatment is known to cause effective excitation-contraction uncoupling, thus minimising the risk of muscle contraction during prolonged recordings. After 10 min in control saline, fish were exposed to a 10 min perfusion with either L-NAME or DETA-NO. During analysis of locomotor-related end plate potentials (EPPs), a low concentration of the neuromuscular blocker tubocurarine (3 µM; Sigma) was substituted for TTX in the extracellular saline. Under these conditions muscle ACh receptors are partially blocked such that muscular contractions are inhibited but locomotor-related drive to muscle fibres can be readily monitored with whole cell patch clamp electrophysiology. Fictive motor episodes were evoked with light stimulation.
During current clamp recordings muscle fibres were injected with a small amount of current to maintain a membrane potential of −75 mV and −65 mV for embryonic fast and slow twitch fibres respectively whereas during voltage clamp experiments, fibres were clamped at −75 mV. Fibres with access resistances 10 MΩ were routinely excluded and series resistance compensated by ≥70%. Biological signals were amplified using an RK-400 amplifier (Biologic) and digitized with a National Instruments A–D converter.
Signals were acquired at a sample rate of 30 KHz and subsequently filtered to 2 kHz offline using a PC running pClamp (Molecular Devices). MEPC and EPP Analysis All analysis was conducted offline in Clampfit (Molecular Devices). Events were detected using the template matching function and all captured events were subject to manual examination where erroneous events were excluded from analysis. MEPC frequency was determined by counting the number of events occurring over a 200 s recording period. Rise times (10–90%) and half-widths of mEPCs were analysed offline using Clampfit. For analysis of locomotor EPPs, 30 EPPs taken from each swim episode were captured using the template matching feature.
These were pooled and averaged and the mean rise time (10–90%), decay time (10–90%) and amplitude recorded. Developmental Effects of Elevated NO/cGMP Levels on NMJ Markers Previously we had shown that NO signalling suppresses zebrafish motor axon branch development and further demonstrated that chronic blockade of NOS (with L-NAME) increases the number of co-localised pre- and post-synaptic domains within the developing zebrafish musculature at 3 days post fertilisation (dpf). However, the effects of NO elevation and sGC/cGMP manipulation on NMJ formation have not been explored. Additionally, as we previously used voxel co-localisation methods to quantify changes in the number of volumetric pixels that contained overlapping synaptic markers, nitrergic effects on synapse number and distribution were not determined. To address these issues we manipulated NO/cGMP levels during development and labelled NMJs with the presynaptic marker anti-SV2 and the postsynaptic ACh receptor marker rhodamine conjugated α-bungarotoxin (Rh-α−BTX). Subsequently we examined the number and spatial distribution of NMJs with SynaptcountJ, a plugin for ImageJ that permits quantification of synapse number (see methods).
Analysis was restricted to the second day of development because by this stage a small, easily quantifiable network of arbours has been established in each somitic region (; ). Since our previous work suggested that NO/cGMP signalling affects motor axon branch formation without impairing motor root growth, we segregated puncta into two domains: those located on the motor axon fascicle and those located on motor axon branches. Using this approach, differential effects of NO/cGMP signalling on each compartment could be examined. To characterise the effects of NO/cGMP signalling on neuromuscular synapse formation, 1 dpf embryos were exposed to embryo medium containing the NO donor DETA-NO or 8-pCPT-cGMP, a cell permeable cGMP analogue. Fish were incubated in drugs until 2 dpf, at which point they were processed for SV2/Rh-α-BTX histochemistry and compared to control fish raised in egg media alone.
In agreement with previous reports, regions of SV2/Rh-α−BTX co-localisation (putative NMJs) were found scattered throughout the muscle of control embryos. When developing embryos were exposed to DETA-NO, a marked decrease in the total number of somitic NMJs was observed (; control = 96.27±5.06, n = 11; DETA-NO = 53.33±2.54, n = 24, p0.05). This effect could arise from NO-dependent control of motor axon branch formation or from a change in the number of NMJs formed per unit of branch length. To distinguish between these possibilities we calculated the density of branch-specific NMJ puncta, finding that DETA-NO had no effect on this parameter (; control = 0.17±0.01 puncta/µm, DETA-NO = 0.16±0.02 puncta/µm, p0.05). This suggests that the NO-dependent reduction in NMJ number is a consequence of the previously reported reduction in motor axon branch formation.
Incubating embryos in 8-pCPT-cGMP had a similar effect to DETA-NO in that fewer NMJ puncta were observed (control = 66.80±2.96, n = 20; 8-pCPT-cGMP = 44.19±2.40, n = 16, p0.05) puncta. Again, this observation could not be accounted for by a change in the density of branch-associated synapses (; control = 0.15±0.01 puncta/µm, 8-pCPT-cGMP = 0.17±0.01 puncta/µm, p0.05).
Collectively these observations suggest that developmental elevation of NO/cGMP reduces the formation of NMJs predominantly through suppression of motor axon branch formation. Developmental Effects of Inhibiting NOS/sGC on NMJ Markers To determine the effects of reducing NO/cGMP levels during development we perturbed either NOS or sGC activity by exposing zebrafish embryos at 24 hpf to L-NAME or ODQ. Fish remained in drugs until 2 dpf, at which point they were processed for immunohistochemistry and compared to age-matched controls. Developmental exposure to L-NAME significantly increased the number of somitic puncta (control = 80.30±4.77, n = 20; L-NAME = 98.56±5.27, n = 23, p0.05) puncta. However, the density of branch-associated puncta was not affected by L-NAME treatment (; control = 0.23±0.03 puncta/µm, L-NAME = 0.19±0.01 puncta/µm, p0.05).
These findings are in agreement with previous observations showing that developmental exposure to L-NAME increases the number of co-localised NMJ markers at 3 dpf. We also found that ODQ treatment increased somitic puncta number (control = 87.5±4.10, n = 18; ODQ = 116.00±5.67, n = 12, p0.05) puncta. Again, we observed no effect on the density of branch-associated puncta (; control = 0.19±0.01 puncta/µm, ODQ = 0.21±0.01 puncta/µm, p0.05). Developmental inhibition of NO/cGMP synthesis increases NMJ numbers. Left hand panels: lateral trunk views of control (top) and L-NAME (bottom) treated zebrafish at 2 days post fertilisation (dpf) processed with anti-SV2 (green) and Rh-α-BTX (red) staining.
Right hand panels: expanded regions showing staining localised to a single somitic region. Bar chart depicting the mean (± SEM) number of synapses located within each somite (som), along motor fascicles (fas) and along branch-associated domains (branch) of control (black) and L-NAME (grey) treated fish. Mean density of branch-associated puncta in control (black) and L-NAME treated (grey) fish. Left hand panels: lateral trunk views of control (top) and ODQ (bottom) treated zebrafish at 2 dpf. Right hand panels: expanded regions showing staining localised to a single somitic region. Bar chart depicting the mean (± SEM) number of synapses located within each somite (som), along motor fascicles (fas) and along branch-associated domains (branch) of control (black) and ODQ (grey) treated fish. Mean density of branch-associated puncta in control (black) and ODQ treated (grey) fish.
Scale bars = 30 µm.p≤0.01,.p≤0.001. Developmental Effects of NO Signalling on mEPP Parameters We next used electrophysiological approaches to determine whether developmental perturbations in NO signalling affected neuromuscular physiology. Two discreet muscle fibre populations, termed ‘embryonic fast’ (EF) and ‘embryonic slow’ (ES) are known to exist in early stage zebrafish,. These differ with respect to speed of contraction, position, orientation, extent of electrical coupling and synaptic kinetics: the ES fibres are superficial, run parallel to the body axis, are extensively electrically coupled to multiple neighbouring ES fibres and generate synaptic currents with relatively slow rise and decay kinetics. By contrast, the EF fibres lie medial to the ES population, are arranged in an oblique orientation, exhibit less extensive electrical coupling and generate synaptic currents with relatively fast kinetics. As a first step towards examining the effects of NO signalling on physiological maturation of zebrafish NMJs we examined the effects of NO on mEPCs occurring in both fibre populations. To this end we synaptically isolated muscle fibres of 2 dpf control, DETA-NO treated and L-NAME treated fish with the sodium channel blocker tetrodotoxin (TTX) and recorded mEPCs in the whole cell voltage clamp configuration,.
During mEPC recordings, the gap junction blocker 18βGA was added to the extracellular saline in an attempt to block gap junction coupling between muscle fibres. Electrical coupling has several effects on muscle physiology. First, it reduces fibre input resistance; second it decreases mEPC amplitude, and; third, it introduces a sub-population of small amplitude, slow currents which are believed to be gap junction filtered representations of mEPCs occurring in neighbouring fibres,. As expected, we found that 18βGA abolished transcellular dye labelling , increased membrane resistance (control EF = 11.76±3.04 MΩ, 18βGA EF = 166.3±19.23 M Ω, p0.6 ms) were observed in recorded muscle fibres.
![Axion dpf 8505pt Axion dpf 8505pt](/uploads/1/2/5/4/125448262/140737966.png)
Based on these observations, we considered these events to be mediated by passage of current through 18βGA insensitive gap junctions and thus excluded them from analysis. Effects of 18βGA on electrical coupling in EF and ES fibres. Images of embryonic fast (EF, A) and embryonic slow (ES, B) muscle fibres that were dialysed with sulforhodamine during whole cell recording. Note that in control saline, dye spreads to neighbouring muscle fibres whilst 18βGA pre-treatment abolishes this effect. Representative traces of miniature end plate currents (mEPCs) occurring in EF ( C) and ES ( D) fibres exposed to 18βGA. Right: example events captured on an expanded time scale.
Amplitude versus rise time plots for mEPCs recorded from EF ( E) and ES ( F) fibres exposed to 18βGA. Insets show histograms of mEPC rise time in control (black) and 18βGA-treated (grey) fibres. Paired recording between ES fibres reveal that coincident events, presumed to be carried by electrical synapses, are occasionally observed in the presence of 18βGA; asterisked events with extended time scale on right.
Scale bars = 50 µM. We next compared mEPCs occurring in EF fibres of control, DETA-NO and L-NAME treated embryos. Cumulative probability distributions indicated that developmental exposure to either DETA-NO or L-NAME reduced mEPC amplitude in EF fibres and analysis of mean amplitudes confirmed that this was the case (; control = 783.80±14.22 pA, n = 47 fibres; DETA-NO = 498.20±20.22 pA, n = 17 fibres; L-NAME = 661.40±13.01 pA, n = 46 fibres; p0.05). Chronic manipulation of NO levels also affected decay kinetics of EF mEPCs. Here, DETA-NO treatment caused a marked prolongation in mEPC half-width (control = 1.207±0.0.019 ms, n = 47 fibres; DETA-NO = 1.859±0.044 ms, n = 17 fibres; p. Developmental perturbation of NO signalling affects EF mEPC kinetics. Average traces of miniature end plate currents (mEPCs) captured during recordings of embryonic fast (EF) fibres in control (left), DETA-NO raised (middle) or L-NAME raised (right) fish at 2 days post fertilisation (dpf).
Cumulative percentage plots and bar charts of EF mEPC amplitude (amp, B,C), rise time (RT, D,E) and half-width (HW, F,G) for each experimental condition. Data in C,E,G are represented as mean ± SEM. p0.05) but was significantly reduced in L-NAME treated fish (control = 634.700±0.19.830 pA, n = 24 fibres; L-NAME = 480.600±13.380 pA, n = 38 fibres; p0.05).
Finally, the half-width of ES mEPCs was prolonged in DETA-NO treated fish (control = 1.239±0.034 ms, n = 24 fibres; DETA-NO = 1.435±0.039 ms, n = 33 fibres; p. Developmental manipulation of NO affects ES mEPC kinetics. Average traces of miniature end plate currents (mEPCs) captured during voltage recordings in embryonic slow (ES) fibres of control (left), DETA-NO raised (middle) and L-NAME raised (right) fish. Cumulative percentage plots and bar charts of ES mEPC amplitude (amp, B,C), rise time (RT, D,E) and half-width (HW, F,G) for each experimental condition.
Data in C,E,G are represented as mean ± SEM. p0.05, data not shown) or ES (control = 0.08±0.01 Hz, n = 24; DETA-NO = 0.06±0.01 Hz, n = 33; L-NAME = 0.08±0.01 Hz, n = 38; p0.05, data not shown) mEPCs. However, during current clamp recordings in which 18βGA was excluded from the bathing media, DETA-NO decreased (control EF = 0.96±0.07 Hz, DETA-NO treated EF = 0.49±0.07 Hz; control ES = 1.26±0.09 Hz, DETA-NO treated ES = 0.95±0.09 Hz, p. Acute Manipulation of NO Levels does not Affect NMJ Physiology To test for acute effects of NO signalling on NMJ physiology we obtained patch recordings from muscle fibres of 2 dpf control fish and subsequently exposed them to a brief (10 min) incubation in either DETA-NO (n = 3 EF, n = 3 ES) or L-NAME (n = 4 EF, n = 3 ES). During recordings , neither bath perfusion of DETA-NO or L-NAME affected the frequency, amplitude, rise time or half-width of EF and ES mEPCs. Thus, transient manipulation of NO signalling does not affect physiological parameters of the developing zebrafish NMJ. Developmental Effects of NO on the Locomotor Drive for Swimming We next asked how developmental perturbation of NO affected the neuromuscular drive for swimming behaviour.
To do this we examined locomotor-related end plate potentials (EPPs) in EF and ES fibres that were not exposed to 18βGA. Using this approach we were thus able to study the NO-dependent regulation of locomotor ontogeny under more physiologically-relevant conditions. The synaptic drive for locomotion was monitored during sensory-evoked bouts of fictive swimming in 2 dpf fish raised in control saline (n = 10 EF; n = 10 ES), DETA-NO (n = 4 EF; n = 4 ES) or L-NAME (n = 7 EF; n = 9 ES). During EF recordings, developmental DETA-NO exposure decreased locomotor EPP amplitudes (; control = 0.94±0.03 mV; DETA-NO = 0.82±0.05 mV, p. Developmental NO manipulation perturbs fictive locomotor drive to ES muscle fibres. Voltage recordings of locomotor-related drive obtained from embryonic slow (ES) fibres of control ( A), DETA-NO raised ( B) and L-NAME raised ( C) fish at 2 days post fertilisation (dpf). Mean end plate potential (EPP) amplitude ( D), rise time ( E) and decay time ( F) measured during episodes of fictive swimming.
Data in D-F are represented as mean ± SEM. NO/cGMP Signalling as a Regulator of Neuromuscular Synaptogenesis Our previous study revealed that NO signalling suppresses and NOS inhibition enhances motor axon branch development in zebrafish. Moreover, voxel analysis revealed that the number of co-localised pre- and post-synaptic NMJ markers is increased by chronic L-NAME exposure. In the current study we have built upon these findings so that the effects of NO and cGMP on NMJ number, density and distribution could be determined. This allowed us to make three important observations. First, we show that developmental elevation of NO/cGMP (via addition of DETA-NO/8-pCPT-cGMP) inhibits, whilst developmental inhibition of NO/cGMP synthesis (via exposure to L-NAME/ODQ) promotes, formation of NMJs within the axial swimming muscles. Second, we show that these effects arise from changes in the number of puncta located on motor axon branches, but not fascicles.
Finally, we show that the density of branch associated-synapses is not affected by NO signalling. Since our previous data demonstrates that NO decreases motor axon branch number, we posit that the NO-dependent modifications in NMJ number reported here arise as a consequence of modified branch formation.
However, once branches are formed, NO does not appear to alter the rate of synapse addition. During the current study we found that NO signalling did not affect the number of fascicular NMJs.
This is perhaps not surprising as drug treatment was initiated at 24 hpf. By this stage, early periods of motor fascicle extension and the incorporation of pre-patterned ACh receptors into fascicular NMJs is complete,. Whilst we cannot exclude the possibility that pharmacological treatment at earlier time points may influence fascicular synaptogenesis, the observation that NOS1 is first expressed within the spinal cord at around 30 hpf strongly suggests that NO has no physiological role during initial stages of neuromuscular development which begins at ∼ 17 hpf. Thus, unlike amphibian and avian species, where NO affects aneural ACh receptor cluster assembly –, the earliest stages of zebrafish NMJ development are unlikely to be NO-dependent. NO Signalling and NMJ Physiology During mEPC experiments we routinely included 18βGA in the bath solution to block electrical synapses within the zebrafish musculature. This drug has previously been reported to abolish the low-amplitude, filtered events that arise from electrotonic spread of current from electrically coupled neighbouring fibres. Here, we found that 18βGA application abolished transcellular spread of sulforhodamine and increased both muscle fibre input resistance and mEPC amplitude.
Although these observations suggest that 18βGA abolished gap junction signalling, we found a population of small, filtered mEPCs persisted in the presence of this drug. Interestingly, Sylvain et al. Reported a similar phenomenon following exposure to the 18βGA analog carbenoxolone, reasoning that they were generated by nascent synapses located on the recorded muscle fibre. However, as we found that similar events often occurred synchronously during paired muscle recordings, we favour the hypothesis that they are caused by current flow through 18βGA-resistant gap junctions. Thus, for the purposes of our study, this population of events was excluded from analysis. Our data suggests that chronic manipulation of NO signalling had marked effects on synaptically-mediated mEPCs. Specifically, developmental elevation of NO (DETA-NO exposure) caused a marked slowing of mEPC rise times and half-widths in EF and ES muscle.
By contrast, mEPC half-widths of L-NAME treated fish were reduced in EF and ES fibres, although rise times were not significantly affected. In addition, L-NAME reduced the amplitude of EF and ES mEPCs whilst DETA-NO reduced EF mEPC amplitudes.
Collectively, these observations suggest that NO may regulate the maturation of immature neuromuscular synapses. In support of this hypothesis, previous studies have shown that the kinetics of zebrafish mEPPs and mEPCs change markedly during neuromuscular ontogeny. During early embryonic development, these exhibit prolonged rise and decay times and relatively small amplitudes. As maturation proceeds, rise and decay times shorten whilst amplitude increases,. The speeding-up of synaptic currents has been attributed to a combination of changes that include maturation of presynaptic properties, the gradual aggregation and addition of ACh receptors at the endplate and the expression of ACh-esterase, which serves to hydrolyse ACh within the synaptic cleft and thus accelerates relaxation of endplate currents –.
In addition, vertebrate neuromuscular ACh receptors often undergo developmentally-related changes in subunit composition which engenders differing kinetic properties during development. For example, immature ACh receptors are typically composed of α2βδγ subunits whilst adult receptors contain α2βδε subunits –. Receptors expressing the γ subunit exhibit prolonged open channel times and generate prolonged macroscopic currents whereas those expressing the ε subunit exhibit short open channel times and generate shorter macroscopic currents.
Whilst a delay in the switch from γ to ε subunits may contribute to the effects of NO on EF fibres, it is unlikely to explain the effects observed within ES fibres as they do not exhibit a developmentally-related change in subunit composition. Future molecular and biochemical studies are needed to identify whether one or more of these developmental phenomena are influenced by NO signalling. Interestingly, we found that the NO-dependent effects on mEPC frequency varied as a function of recording conditions.
In the absence of 18βGA, event frequency decreased in DETA-NO treated fish but increased in L-NAME treated fish. When considered in light of our anatomical findings, we hypothesise that these effects are caused by NO-dependent regulation of NMJ numbers. By contrast, differences in mEPC frequency were not observed when 18βGA was included in the extracellular saline. These effects could arise because differences in mEPC frequency may only be apparent when inputs to multiple muscle fibres are simultaneously monitored. Addition of 18βGA would reduce electrical coupling between fibres, thus reducing the number of fibres from which events could be sampled. Developmental Effects of NO on Locomotor Drive Although transient NO biosynthesis is known to modulate parameters of ongoing locomotor activity, its relevance to locomotor maturation has remained unclear. Here we show that chronic manipulation of NO signalling can strongly affect locomotor network output.
Developmental exposure to DETA-NO prolongs the rise and decay durations of locomotor-related EPPs. Whilst the effects of NOS inhibition (L-NAME incubation) were less pronounced, we did observe a small acceleration in EF/ES EPP rise times and a reduction in ES EPP decay times during locomotor activity. Given that mEPC rise and decay times are prolonged in DETA-NO and accelerated in L-NAME, the observed changes to the neuromuscular drive may, at least in part, arise from a change in the waveform of macroscopic currents generated by ACh receptor activation. Specifically, DETA-NO dependent slowing of the rise and decay time of ACh receptor currents would be expected to slow rates of membrane depolarisations and repolarisations during swimming.
By contrast, L-NAME, which accelerated the rise and decay times of mEPCs would be expected to have the opposite effect. However, other factors may also contribute to this phenomenon. For example, the extensive electrical coupling between zebrafish embryo muscle fibres is known to filter postsynaptic responses within the muscle,. Thus, a change in the extent of electrical coupling could affect the rise and decay times of EPPs recorded during swimming.
Additionally, the amplitude, rise and decay time of compound EPPs is known to be influenced by the time course of transmitter release from individual presynaptic terminals,. A number of factors, including synaptic ACh, catecholamines and presynaptic action potential duration, have been shown to influence release latency at the NMJ and as a consequence affect the rise, decay and amplitude of postsynaptic events. On this note, NO has been reported to directly affect ACh release at the NMJ, catecholamine release within the spinal cord and neuronal excitability of interneuron populations and so could potentially alter release latency via a number of different mechanisms.
Future motoneuron-muscle recordings will help to determine whether NO does indeed have an effect on the timecourse of evoked ACh release at the developing NMJ. We also found that when compared to controls, the frequency of locomotor-related EPPs was lower in DETA-NO treated and higher in L-NAME treated fish, an observation that stands in broad agreement with our previous behavioural studies. In addition, we observed that both DETA-NO and L-NAME exposure reduced the duration of locomotor-related episodes. Although the mechanism of action awaits investigation, this effect is likely to be caused by an NO-dependent effect upon the properties of spinal cord interneurons or descending motor control pathways, rather than NO-dependent regulation of NMJ properties: at the onset of swimming (approximately 26 hpf), the locomotor network contains a small number of immature neurons that can only generate low-frequency, inflexible forms of swimming behaviour,. As electrical properties of spinal neurons mature and new cells are integrated into the spinal network, fish become capable of swimming at a wide range of frequencies –.
Thus, an NO-dependent effect on the development of later-developing spinal interneurons may underlie the observed actions of NO. Alternatively, observed changes in motor output might arise from homeostatic feedback mechanisms that scale the frequency of rhythm generator activity to the biophysical characteristics of the muscle. Thus, the NO-dependent slowing of postsynaptic NMJ kinetics may trigger feedback mechanisms that ensure network activity is limited to frequencies that do not cause EPP summation and tetanus. In contrast, the speeding up of postsynaptic responses in conditions of low NO may allow the locomotor network to drive muscles at slightly higher frequencies. Irrespective of the causes, our observations stand in agreement with previous behavioural studies showing a reduction in swim frequency in fish exposed to NO donors during development and an acceleration in locomotor output in fish lacking the NOS1 isozyme. In summary, our data provide strong evidence that NO serves as a developmental regulator of NMJ maturation in zebrafish.
These observations provide further support for the hypothesis that NO is a fundamentally important signalling molecule during periods of NMJ synaptogenesis and locomotor maturation.